The center is planned for construction just east of Riverside Road off Highway 36.
The Missouri Department of Transportation (MoDOT) has already agreed to build a new highway interchange at the site.
A group of 18 volunteers is looking at a goal of raising $5 million locally.
They say there is a wide range of people who should be interested in contributing to the Ag Expo.
"We believe anyone who is committed to the life sciences, to agribusiness, to business itself, entrepreneurs, commercial interests will benefit from the Expo Center," said Melody Smith, the campaign chair for the center.
"How we can help that farmer out there with some new technology and new opportunities?" said Sharon Cornelius, Expo Center President and CEO.
"That agribusiness person who is just needing a place to get started and going, for conferences and those things related to agriculture that brings to this region and really to the whole world. We help feed the world," she said.
About half of the $5 million local goal has already been met.
Staff hopes to see construction at the Ag Expo Center begin before the end of the year.