They say they are disappointed by the outcome of the election, but say they respect the voter's decision.
The project would have paid for more than 800 highway and transportation projects over the next decade.
District Engineer Don Wichern says going forward MoDOT will continue to do the best with what they have.
"What we've continued to do for many years and it's been our goal forever is we're gonna take care of the system and keep it as good as we can for as long as we can with the resources that we have to spend," he said.
Wichern says drivers in Missouri will not see much of a change for the next couple of years, but by 2017, they will not be able to maintain the roads they have.
He says it is out of their hands on if they will try to get the issue on the ballot again in the future.